WebDownload scientific diagram RRM2-c2orf48 enhances cancer cell migration and NPC invasion. (a) Establishment of stable cell lines of overexpressed RRM2-c2orf48 and confirmed by western blotting ... WebCloning the open reading frame (ORF) of a protein-coding chimeric RNA into a mammalian expression vector is a simple, practiced way to study the function of the chimeric RNA. Here, we provide procedures for the insertion of the RRM2-C2orf48 fusion ORF into a mammalian expression vector, achieving th …
C2orf48 - PrimePCR Assay and Template Life Science Bio-Rad
WebDownload scientific diagram Representative IHC staining of RRM2-c2orf48 in NPC specimens. (a) Nearly negative expression of RRM2-c2orf48; intensity level scored as 0. (b) Weak expression of RRM2 ... Web丁香通为您提供Adamts10商品详情介绍：价格：询价，货号：GC-Mm24244，NCBI推荐的基因克隆供应商 产品优势 多——140,000+个ORF克...详见丁香通Adamts10商品详情页； honeyville vital wheat gluten
Landscape characterization of chimeric RNAs in colorectal cancer.
WebMay 5, 2024 · Our ceRNA network showed C2orf48 and LINC00483 might upregulate CCNB1 via competing with miR‐183. In addition, we found a subnetwork which contained survival‐associated DERNAs (AC110491.1, LINC00483‐miR‐192‐GRHL1). The results of reverse transcription quantitative polymerase chain reaction supported the relative … Cells with overexpression of RRM2-c2orf48 and vector were seeded on coverslips in 24-well plates at a density of 5 × 104cells per well for 18–24 h. They were briefly washed with PBS twice, fixed with 3% paraformaldehyde in PBS for 20 min, and then permeabilized with 0.1% Triton × 100 in PBS for 5 min. After … See more Total RNA was extracted from the NPC cell line, C666-1 and NPEC2. Beads bound to oligo(dT) were used to isolate poly(A) mRNA from total RNA. cDNA libraries were … See more Sixty NPC specimens and 20 noncancerous epithelial tissues used in studies of RRM2-c2orf48 expression based on qRT-PCR … See more Total RNA from different cell lines and human tissues was extracted using Trizol reagent (Invitrogen). RNA concentrations and quantities were … See more NPECs were immortalized by transfecting Bmi-1 as described previously and were cultured in keratinocyte serum-free medium (Invitrogen, Carlsbad, CA, USA)31 and labeled NPEC1 Bmi-1 and NPEC2 Bmi-1. … See more WebDec 1, 2001 · C2orf48. Organism names. Organism. Homo sapiens (Human) Taxonomic identifier. 9606 NCBI. Taxonomic lineage. Eukaryota > Metazoa > Chordata > Craniata > … honeyville powdered whole eggs