Web注意：DH10Bac菌株携带⼀个昆⾍杆状病毒穿梭载体（bMON14272，136 kb）和⼀个辅助质粒（pMON7142，13.2 kb），能够⽀持昆⾍杆状病毒转移载体pBV和bMON14272两者 … WebDH10Bac 细胞包含一种杆状病毒穿梭载体 (bMON14272) 和一种辅助性质粒 (pMON7142)，能够支持 pFastBac 与 bMON14272 的位点特异性重组以生成高分子量杆 …

Invitrogen™ MAX Efficiency™ DH10Bac Competent Cells

Webbaculovirus shuttle vector (bMON14272)와 helper plasmid (pMON7142)를 함유하고 있어 pFastbac plasmid와 bMON14272 사이의 site-specific recombination를 유도하여 bacmid를 형성함. 이 bacmid는 insect cell transfection에 이용됨. hsdR: type I restriction enzyme (random dsDNA 절단, 변이로 기능상실). WebDH10Bac-Zellen beherbergen einen Baculovirus-Shuttle-Vektor (bMON14272) und ein Helferplasmid (pMON7142) und können Bacmide mit hohem Molekulargewicht erzeugen, die dann verstärkt, aufgereinigt und für Insektenzelltransfektion und nachfolgende Genexpression verwendet werden können. trefoil in architecture

Can anyone provide me the sequence of the baculovirus shuttle …

WebDH10Bac cells harbor a baculovirus shuttle vector (bMON14272) and a helper plasmid (pMON7142), and are capable of supporting site-specific recombination between pFastBac and bMON14272 to generate high … WebThe current presence of the helper plasmid (pMON7142) helping site-specific recombination between your generating build pFBD-polh-TYLCV-CP and bMON14272 to create high … WebDH10Bac cells harbor a baculovirus shuttle vector (bMON14272) and a helper plasmid (pMON7142), and are capable of supporting site-specific recombination between pFastBac and bMON14272 to generate high molecular weight bacmids that can then be amplified, purified, and used for insect cell transfection and subsequent gene expression. trefoil house wroughton